Probiotic Bacteria against resistant-carpabenem Acinetobacter baumennii
Infections caused by carbapenem resistance Acinetobacter baumannii (crab) are continually a focus of significant attention since the limitation of therapeutic options. Probiotic bacteria always have an essential role in dairy products and fermented food and promoted the positive health image as the treatment of microbial infections.
Here, we evaluated the potential of antimicrobial agent produced by probiotics for the protection against crab infection. Tracheal aspirate specimen from 150 patients at Egyptian hospitals were recognized as Ab by PCR detection of blaOXA-51.
Antimicrobial susceptibility was studied. Positive crab isolates with blaOXA-24 and blaOXA-58incidence were undergoing for screening using probiotics that have been isolated from dairy products & food supplement. Probiotic have highest antagonistic activity was identified and its bioactive compounds were purified & characterized by studying physiochemical characters.
Nearly ninety-six of the cases were crab & 37.5% of cases harboring blaOXA-24 & only one case has blaOXA-58. In vitro, significantly 80% (P<0.05) of crab remarkably inhibited by four probiotics. Bifidobacterium bifidum strain that showed the highest activity against crab has been identified, with significant inhibition levels reaching 83.33% in the case of the supernatant and even 97% inhibition of supernatant purified by column chromatography. Purified BbV1 was heat stable with amino acid content as identified by LC-MS/MS and belonged to bacteriocins-like compounds. Our finding demonstrated that natural BbV1 provides a protection against Acinetobacter infection in vitro. In vivo, further studies were applied using immunological and histological studies for application as nutritional and pharmaceutical use.
Probiotic bacteria have many beneficial health effects, and one of them is their ability to bind aflatoxin. Evidences from in vitro, animal and human clinical studies have supported the potential ability of probiotic bacteria as adsorbent of aflatoxin. Although the mechanism of which probiotic bacteria bind to aflatoxin is unclear and literature on the mechanism is scarce, a lot of studies have found promising and fruitful outcomes. It is important to study the chemical interactions between the cell wall of probiotic bacteria, and its related components with aflatoxin molecules as such data can provide further justification of probiotics as adsorbent of aflatoxin. As functional foods, probiotic bacteria are of a great interest as human exposure to aflatoxin is ubiquitous and its application as one of the dietary approaches to prevent human exposure to this food-borne contaminant is significant. In many developing countries such as in Africa and Asia, dietary aflatoxin exposure is incessant and prevalent. Therefore, well-developed studies on probiotic bacteria as a preventive agent are warranted, where its application for humans can eventually be practiced in order to minimize aflatoxin exposure and improve the overall health.
Microbiology: Current Research